作者
Hoang Le Tuan Anh, Dong-Cheol Kim, Wonmin Ko, Tran Minh Ha, Nguyen Xuan Nhiem, Pham Hai Yen, Bui Huu Tai, Luu Hong Truong, Vu Ngoc Long, Tran Gioi, Tran Hong Quang, Chau Van Minh, Hyuncheol Oh, Youn-Chul Kim, Phan Van Kiem
发表日期
2017/1/1
期刊
Pharmaceutical biology
卷号
55
期号
1
页码范围
1195-1201
出版商
Taylor & Francis
简介
Context: Paramignya trimera (Oliv.) Burkill (Rutaceae) has been used to treat liver diseases and cancer. However, the anti-inflammatory effects of this medicinal plant and its components have not been elucidated.
Objective: This study investigated chemical constituents of the P. trimera stems and evaluated anti-inflammatory effects of isolated compounds.
Materials and methods: Cytotoxicity of isolated compounds (5–40 μM) toward BV2 cells was tested using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) for 24 h. Inhibitory effects of isolated compounds (5-40 μM) on nitrite and PGE2 concentrations were determined using Griess reaction and PGE2 ELISA kit, respectively (pretreated with the compounds for 3 h and then stimulated for 18 h with LPS). Inhibitory effects of compounds (5-40 μM) on iNOS and COX-2 protein expression were evaluated by Western blot analysis (pretreated with the …
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HL Tuan Anh, DC Kim, W Ko, TM Ha, NX Nhiem… - Pharmaceutical biology, 2017