作者
Henry HQ Heng, Sandra Goetze, Christine J Ye, Guo Liu, Joshua B Stevens, Steven W Bremer, Susan M Wykes, Juergen Bode, Stephen A Krawetz
发表日期
2004/3/1
期刊
Journal of cell science
卷号
117
期号
7
页码范围
999-1008
出版商
Company of Biologists
简介
The biological significance of nuclear scaffold/matrix-attachment regions (S/MARs) remains a topic of long-standing interest. The key to understanding S/MAR behavior relies on determining the physical attributes of in vivo S/MARs and whether they serve as rigid or flexible chromatin loop anchors. To analyze S/MAR behavior, single and multiple copies of the S/MAR-containing constructs were introduced into various host genomes of transgenic mice and transfected cell lines. These in vivo integration events provided a system to study the association and integration patterns of each introduced S/MAR. By utilizing FISH to visualize directly the localization of S/MARs on the nuclear matrix or chromatin loop, we were able to assign specific attributes to the S/MAR. Surprisingly, when multiple-copy S/MARs were introduced they were selected and used as nuclear matrix anchors in a discriminatory manner, even though …
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