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The shoot regeneration response from different explants, namely cotyledon, cotyledonary node and petiole, of the most popular Indian cultivar, JL-24, of groundnut, was evaluated by culturing on MS medium containing B₅ vitamins supplemented with different growth regulators. Shoot bud initiation, proliferation and subsequent development into shoots were superior from cotyledonary node explants compared to the other explants. High frequency (69%) direct shoot regeneration from cotyledonary node was observed on MS medium with 4.0 mg benzyladenine and 0.2 mg NAA/litre. The shoots elongated upon transfer to MS medium with lower concentrations of benzyladenine. Elongated shoots could be continuously harvested at 15 days interval for a period of 75 to 90 days. The regenerated shoots were rooted at a high frequency of 84.5% on MS medium containing 1.0 mg NAA/litre. The regenerated plantlets …