作者
Pei-Hsun Wu, Dikla Raz-Ben Aroush, Atef Asnacios, Wei-Chiang Chen, Maxim E Dokukin, Bryant L Doss, Pauline Durand-Smet, Andrew Ekpenyong, Jochen Guck, Nataliia V Guz, Paul A Janmey, Jerry SH Lee, Nicole M Moore, Albrecht Ott, Yeh-Chuin Poh, Robert Ros, Mathias Sander, Igor Sokolov, Jack R Staunton, Ning Wang, Graeme Whyte, Denis Wirtz
发表日期
2018/7
期刊
Nature methods
卷号
15
期号
7
页码范围
491-498
出版商
Nature Publishing Group US
简介
The mechanical properties of cells influence their cellular and subcellular functions, including cell adhesion, migration, polarization, and differentiation, as well as organelle organization and trafficking inside the cytoplasm. Yet reported values of cell stiffness and viscosity vary substantially, which suggests differences in how the results of different methods are obtained or analyzed by different groups. To address this issue and illustrate the complementarity of certain approaches, here we present, analyze, and critically compare measurements obtained by means of some of the most widely used methods for cell mechanics: atomic force microscopy, magnetic twisting cytometry, particle-tracking microrheology, parallel-plate rheometry, cell monolayer rheology, and optical stretching. These measurements highlight how elastic and viscous moduli of MCF-7 breast cancer cells can vary 1,000-fold and 100-fold …
学术搜索中的文章
PH Wu, DRB Aroush, A Asnacios, WC Chen… - Nature methods, 2018