作者
Mirna Brkljačić, Vesna Matijatko, Ivana Kiš, Nada Kučer, Jadranka Foršek, Renata Rafaj, Darko Grden, Marin Torti, Iva Mayer, Vladimir Mrljak
发表日期
2010/3/1
期刊
Acta Veterinaria Hungarica
卷号
58
期号
1
页码范围
39-46
出版商
Akadémiai Kiadó
简介
The aim of the present study was to detect and characterise the species and subspecies of Babesia spp. that cause canine babesiosis in Croatia. Twenty-eight dogs with typical signs of babesiosis (lethargy, anorexia, fever, dark urine and thrombocytopenia) were included in this study. Their blood smears showed the presence of Babesia canis . The results showed the detection of one subspecies, namely Babesia canis canis using PCR, and subsequent sequence analysis demonstrated portions of the nss rRNA gene in 27 out of 28 samples. Sequence analysis of the isolates showed 100% identity in 11 samples, 99.7% identity (one nucleotide difference) in 11 samples and 99.4% identity (two nucleotides difference) in 5 samples with B. canis canis . The results of this study confirm the presence of B. canis canis in infected dogs in Croatia and …
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M Brkljačić, V Matijatko, I Kiš, N Kučer, J Foršek… - Acta Veterinaria Hungarica, 2010