作者
Jie Hu, Xuejing Jia, Xiaobin Fang, Peng Li, Chengwei He, Meiwan Chen
发表日期
2016/4/30
期刊
International journal of biological macromolecules
卷号
85
页码范围
277-284
出版商
Elsevier
简介
Ultrasonic-assisted extraction technology was employed to prepare Ligusticum chuanxiong Hort polysaccharide. Single factor test and orthogonal experimental design were used to optimize the extraction conditions. The results showed that the optimal extraction conditions consisted of ultrasonic temperature of 80 °C, ultrasonic time of 40 min and water to raw material ratio of 30 mL/g. Three novel polysaccharides fractions, LCX0, LCX1 and LCX2, were isolated and purified from the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography. The molecular weight and monosaccharide composition of three LCX polysaccharides fractions were analyzed with gel permeation chromatography (GPC) and HPLC analysis, respectively. Furthermore, the antioxidant and in vitro anticancer activities of the polysaccharides were investigated. Compared with LCX0, LCX2 and LCX1 showed …
学术搜索中的文章
J Hu, X Jia, X Fang, P Li, C He, M Chen - International Journal of Biological Macromolecules, 2016