作者
Julia A Beaver, Danijela Jelovac, Sasidharan Balukrishna, Rory L Cochran, Sarah Croessmann, Daniel J Zabransky, Hong Yuen Wong, Patricia Valda Toro, Justin Cidado, Brian G Blair, David Chu, Timothy Burns, Michaela J Higgins, Vered Stearns, Lisa Jacobs, Mehran Habibi, Julie Lange, Paula J Hurley, Josh Lauring, Dustin A VanDenBerg, Jill Kessler, Stacie Jeter, Michael L Samuels, Dianna Maar, Leslie Cope, Ashley Cimino-Mathews, Pedram Argani, Antonio C Wolff, Ben Ho Park
发表日期
2014/5/15
期刊
Clinical cancer research
卷号
20
期号
10
页码范围
2643-2650
出版商
American Association for Cancer Research
简介
Purpose: Detecting circulating plasma tumor DNA (ptDNA) in patients with early-stage cancer has the potential to change how oncologists recommend systemic therapies for solid tumors after surgery. Droplet digital polymerase chain reaction (ddPCR) is a novel sensitive and specific platform for mutation detection.
Experimental Design: In this prospective study, primary breast tumors and matched pre- and postsurgery blood samples were collected from patients with early-stage breast cancer (n = 29). Tumors (n = 30) were analyzed by Sanger sequencing for common PIK3CA mutations, and DNA from these tumors and matched plasma were then analyzed for PIK3CA mutations using ddPCR.
Results: Sequencing of tumors identified seven PIK3CA exon 20 mutations (H1047R) and three exon 9 mutations (E545K). Analysis of tumors by ddPCR confirmed these mutations and …
学术搜索中的文章
JA Beaver, D Jelovac, S Balukrishna, RL Cochran… - Clinical cancer research, 2014