作者
Claudia Schneider, Cindy L Will, Olga V Makarova, Evgeny M Makarov, Reinhard Lührmann
发表日期
2002/5/1
期刊
Molecular and cellular biology
出版商
Taylor & Francis
简介
In the U12-dependent spliceosome, the U4atac/U6atac snRNP represents the functional analogue of the major U4/U6 snRNP. Little information is available presently regarding the protein composition of the former snRNP and its association with other snRNPs. In this report we show that human U4atac/U6atac di-snRNPs associate with U5 snRNPs to form a 25S U4atac/U6atac. U5 trimeric particle. Comparative analysis of minor and major tri-snRNPs by using immunoprecipitation experiments revealed that their protein compositions are very similar, if not identical. Not only U5-specific proteins but, surprisingly, all tested U4/U6-and major tri-snRNP-specific proteins were detected in the minor tri-snRNP complex. Significantly, the major tri-snRNP-specific proteins 65K and 110K, which are required for integration of the major tri-snRNP into the U2-dependent spliceosome, were among those proteins detected in the …
引用总数
学术搜索中的文章
C Schneider, CL Will, OV Makarova, EM Makarov… - Molecular and cellular biology, 2002