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The present study aimed to examine the influence of leg dominance on the change of direction (COD) performance. In this study participated 94 healthy young highly trained male soccer players belonging from two categories (n = 27 vs. n = 67; 14.81 ± 0.40 vs. 16.64 ± 1.25 years of age; 170.61 ± 5.61 vs. 173.73 ± 7.19 cm of height; 64.74 ± 8.44 vs. 66.70 ± 7.95 kg of weight, for U16 and U18, respectively). Fitness assessments were performed two times in a period of three months, and included: (1) anthropometry measures, (2) 30–15 IFT, (3) 10-m sprint test, (4) 505-COD test, 90° COD test and cross-over hop test. A paired sample t-test was performed to evaluate the asymmetries at the intragroup level in each of the COD’s tests. A symmetry index was used to analyse the asymmetries between categories, and an independent sample t-test was used to compare the variability between the two …