作者
Emmanuelle Charafe-Jauffret, Christophe Ginestier, Flora Iovino, Carole Tarpin, Mark Diebel, Benjamin Esterni, Gilles Houvenaeghel, Jean-Marc Extra, François Bertucci, Jocelyne Jacquemier, Luc Xerri, Gabriela Dontu, Giorgio Stassi, Yi Xiao, Sanford H Barsky, Daniel Birnbaum, Patrice Viens, Max S Wicha
发表日期
2010/1/1
期刊
Clinical cancer research
卷号
16
期号
1
页码范围
45-55
出版商
American Association for Cancer Research
简介
Purpose: To examine the role of cancer stem cells (CSC) in mediating metastasis in inflammatory breast cancer (IBC) and the association of these cells with patient outcome in this aggressive type of breast cancer.
Experimental Design: CSCs were isolated from SUM149 and MARY-X, an IBC cell line and primary xenograft, by virtue of increased aldehyde dehydrogenase (ALDH) activity as assessed by the ALDEFLUOR assay. Invasion and metastasis of CSC populations were assessed by in vitro and mouse xenograft assays. Expression of ALDH1 was determined on a retrospective series of 109 IBC patients and this was correlated with histoclinical data. All statistical tests were two sided. Log-rank tests using Kaplan-Meier analysis were used to determine the correlation of ALDH1 expression with development of metastasis and patient outcome.
Results: Both in vitro and xenograft …
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E Charafe-Jauffret, C Ginestier, F Iovino, C Tarpin… - Clinical cancer research, 2010