作者
Fatemeh Dehghani, Mostafa Haji Molla Hoseini, Arash Memarnejadian, Farshid Yeganeh, Alireza Mokarram Rezaie, Vahid Khaze, Mandana Sattari, Hassan Darbandi Tamijani, Farzaneh Labibi, Nariman Mossaffa
发表日期
2011/10/1
期刊
Archives of Medical Research
卷号
42
期号
7
页码范围
572-576
出版商
Elsevier
简介
BACKGROUND
Chitin microparticles (CMPs) are found to be potent macrophage stimulators; however, their immunomodulatory effects on the parasite-infected macrophages have not yet been studied. To address this issue, we used a Leishmania major-infected murine macrophage model and characterized the regulatory effects of CMPs on the parasite-infected cells.
METHODS
Mouse peritoneal macrophages were prepared and infected with L. major (MRHO/IR/1975/ER) standard strain. Following cell treatment with CMPs (500 μg/mL) for 48 h, percent of infected macrophages was determined by Giemsa staining and compared with untreated cells. To find the potential mechanisms of the activity of CMPs, TNF-α and accumulated nitrite in the culture supernatants of the treated and untreated cells were also measured by ELISA and colorimetric Griess assays, respectively.
RESULTS
According to the obtained …
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Immunomodulatory activities of chitin microparticles on leishmania major-infected murine macrophages
F Dehghani, MHM Hoseini, A Memarnejadian… - Archives of Medical Research, 2011