作者
Shirish Shukla, Sutapa Mahata, Gauri Shishodia, Shailja Pande, Gaurav Verma, Suresh Hedau, Suresh Bhambhani, Archana Kumari, Swaraj Batra, Seemi F Basir, Bhudev C Das, Alok C Bharti
发表日期
2014/4/1
期刊
Indian Journal of Medical Research
卷号
139
期号
4
页码范围
531-543
出版商
Medknow
简介
Methods:
Both, viral load and integration status of HPV16 were determined by real time URR PCR and estimation of E2: E6 ratio in a total of 130 PGMY-RLB-confirmed, monotypic HPV16-infected cervical DNA samples from biopsies of cytology-confirmed low grade (LSIL, 30) and high grade (HSIL, 30), and invasive carcinoma,(squamous cell carcinoma SCC, 70) cases.
Results:
Investigation of DNA samples revealed a gradual increase in HPV16 viral load over several magnitudes and increased frequency of integration from LSIL to HSIL and HSIL to invasive cancer in relation to the severity of lesions in monotypic HPV16-infected cervical tissues. In a substantial number of precancer (11/60) and cancer cases (29/70), HPV16 was detected in concomitant mixed form. The concomitant form of HPV16 genome carried significantly higher viral load.
Interpretation & conclusions:
Overall, viral load and integration increased …
学术搜索中的文章
S Shukla, S Mahata, G Shishodia, S Pande, G Verma… - Indian Journal of Medical Research, 2014