作者
MR Hoda, SJ Keely, LS Bertelsen, WG Junger, D Dharmasena, KE Barrett
发表日期
2007/3
期刊
British Journal of Surgery
卷号
94
期号
3
页码范围
346-354
出版商
John Wiley & Sons, Ltd.
简介
Background
Obesity is associated with increased levels of leptin. The mitogenic actions of leptin have been identified in various cell types. Because obesity may be a risk factor for colonic cancer, the proliferative and antiapoptotic effects of leptin on colonic cancer cells and the role of mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3-K) signalling were investigated.
Methods
Three human colonic cancer cell lines (T84, HT29/Cl.19A and Caco-2) were treated with leptin. Cell proliferation was measured using the XTT® colorimetric assay and apoptosis by a cell death enzyme-linked immunosorbent assay. Inhibitors of MAPK and PI3-K were used to evaluate the role of these signalling pathways. Phosphorylation of the downstream components extracellular signal-regulated kinase (ERK) 1/2 and Akt was detected by western blotting …
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