作者
Fanghua Liu, Amelia-Elena Rotaru, Pravin M Shrestha, Nikhil S Malvankar, Kelly P Nevin, Derek R Lovley
发表日期
2012
期刊
Energy & Environmental Science
卷号
5
期号
10
页码范围
8982-8989
出版商
Royal Society of Chemistry
简介
Granular activated carbon (GAC) is added to methanogenic digesters to enhance conversion of wastes to methane, but the mechanism(s) for GAC's stimulatory effect are poorly understood. GAC has high electrical conductivity and thus it was hypothesized that one mechanism for GAC stimulation of methanogenesis might be to facilitate direct interspecies electron transfer (DIET) between bacteria and methanogens. Metabolism was substantially accelerated when GAC was added to co-cultures of Geobacter metallireducens and Geobacter sulfurreducens grown under conditions previously shown to require DIET. Cells were attached to GAC, but did not aggregate as they do when making biological electrical connections between cells. Studies with a series of gene deletion mutants eliminated the possibility that GAC promoted electron exchange via interspecies hydrogen or formate transfer and demonstrated that …
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F Liu, AE Rotaru, PM Shrestha, NS Malvankar… - Energy & Environmental Science, 2012