作者
Willemijn van den Ancker, Theresia M Westers, David C de Leeuw, Yvonne FCM van der Veeken, Anne Loonen, Ernest van Beckhoven, Gert J Ossenkoppele, Arjan A van de Loosdrecht
发表日期
2013/3
期刊
Cytometry Part B: Clinical Cytometry
卷号
84
期号
2
页码范围
114-118
出版商
Wiley Subscription Services, Inc., A Wiley Company
简介
Background
According to WHO 2008 guidelines, an important role is designated for cytoplasmic myeloperoxidase (cMPO) as measured by flow cytometry for classifying acute leukemia of myeloid or ambiguous origin (AML or MPAL). However, no threshold with respect to expression level and percentage positive cells is provided. Since the expression of solely cMPO can change the diagnosis from acute lymphoid leukemia into MPAL in the current WHO 2008, a consensus is needed for the cut‐off for cMPO.
Methods
In this study, we investigated whether or not a cut‐off of 10% positivity for cMPO equally defines an acute leukemia as AML or MPAL as compared to a cut‐off for cMPO of 20% and compared this with results obtained for Sudan Black B (SBB) staining by cytomorphology.
Results
Cell lineage‐defining markers and SBB staining were analyzed retrospectively in a cohort of 198 patients who presented …
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W van den Ancker, TM Westers, DC de Leeuw… - Cytometry Part B: Clinical Cytometry, 2013