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Transglutaminase type II (TG2) is a pleiotropic enzyme that exhibits various activities and it is involved in diverse biological functions, including cell signaling, cytoskeleton rearrangements, displaying enzymatic activities. We previously demonstrated that TG2 may contribute to angiotensin II-induced reduction of NO bioavailability as well as to the impaired vascular functional and structural alterations induced by angiotensin II. Here we hypothesized that TG2 may contribute to increased production of reactive oxygen species (ROS) in the vasculature of angiotensin-II-treated mice. TG2-knockout mice (TG2-K/O, 8 weeks old, n=6) and age-matched wild type (WT) control mice were treated or not with angiotensin-II (400ng/kg/min) for 14 days. TG2 activity in aorta was measured by ELISA. ROS production in aorta was evaluated by dihydroethidium staining. The expression of angiotensin type1 receptor (AT1R), TG2, NOX …