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Azoospermia factor locus (AZF) is assumed to contain the genes responsible for spermatogenesis. Deletions in these genes are thought to be pathologically involved in some cases of male infertility associated with azoospermia or oligozoospermia. An attempt was made to establish the prevalence of micro-deletions on the Y chromosome in 180 Environmental Tobacco Smokers [ETS] Tamil Nadu, India. Polymerase chain reaction (PCR) micro-deletion analysis was done in 180 EST males including control. For this, genomic DNA was extracted from the peripheral blood. Seven sets of STS primers were used to amplify the samples encompassing AZFa, AZFb and AZFc regions.