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To identify the larvicidal activity of the volatile oil from Sesuvium portulacastrum (S. portulacastrum) against Anopheles stephensi and Aedes aegypti. Volatile oil extract of S. portulacastrum was prepared in a graded series of concentration. The test for the larvicidal effect of volatile oil against mosquitos larvae was conducted in accordance with the WHO standard method. Batches of 25 early 4th instar larvae of two mosquitoes were transferred to 250 mL enamel bowl containing 199 mL of distilled water and 1 mL of plant extracts. Each experiment was conducted with triplicate with concurrent a control group. Volatile oil extract of S. portulacastrum showed toxicity against 4th instar larvae of Aedes aegypti and Anopheles stephensi with equivalent LC50 value [(63 依 7. 8) µL/mL, LCL-UCL= 55. 2-64. 0] and LC90 value [(94. 2 依 3. 9) µL/mL)] in maximum activity with minimum concentration (200 µL/mL) of volatile oil and followed by maximum activity of 250 µL concentration showed LC50 value=(68. 0 依 8. 2) µL/mL, LCL-UCL= 66. 26-69. 2 and LC50 value of (55. 2 依 2. 8) µL/mL, LCL-UCL= 53. 7-56. 9, LC90=(95. 2 依 1. 25) µL/mL and followed by 250 µL of oil extract against 4th instar larvae of Aedes aegypti respectively. It is inferred from the present study that, the extracts from salt marsh mangrove plan of S. portulacastum are identified as a potential source of safe and efficacious mosquito control agents for the management of vector borne diseases of malaria and dengue.