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Zinc oxide nanoparticles (ZnO NPs) have emerged as a good anticancer and antibacterial activity, which are involved with their strong ability to trigger excess reactive oxygen species (ROS) production, release zinc ions and induce cell apoptosis. The present study aims to synthesize ZnO nanoparticles from zinc acetate (chemical co-precipitation method), and green synthesis (Sesbania grandiflora leaf extract) method evaluated their antibacterial and hemolytic activities. The prepared ZnO NPs were characterized by various techniques such as UV-visible absorption spectroscopy, X-ray diffraction (XRD), field emission scanning electron microscope (FE-SEM), energy dispersive X-ray analysis (EDX), high-resolution transmission electron microscope (HR-TEM) and Fourier transform infrared spectroscopy (FTIR). FE-SEM study reveals that the morphology of the crystal is spherical and flakes-shaped and the …