查看文章

Expression of theobgene and production of leptin have been examined on differentiation of rat fibroblastic preadipocytes to adipocytes in primary culture. Preadipocytes were obtained from the inguinal fat pad of suckling rats, and following differentiation the cells contained lipid droplets and the mRNAs for both lipoprotein lipase and adipsin were detected by Northern blotting.obmRNA was not, however, detected on Northern blots, but analysis by RT-PCR indicated that theobgene was expressed, particularly after differentiation. Measurement of leptin in the culture medium by ELISA showed that theobgene product was secreted by adipocytes from approximately 4 days after the induction of differentiation. Leptin production was sustained over a 2-week period with a peak at 8-10 days post-induction. Dexamethasone stimulated leptin production, while an inhibition was observed with the β-adrenoceptor agonist …