作者
M Kyle Cromer, Sriram Vaidyanathan, Daniel E Ryan, Bo Curry, Anne Bergstrom Lucas, Joab Camarena, Milan Kaushik, Sarah R Hay, Renata M Martin, Israel Steinfeld, Rasmus O Bak, Daniel P Dever, Ayal Hendel, Laurakay Bruhn, Matthew H Porteus
发表日期
2018/10/3
期刊
Molecular Therapy
卷号
26
期号
10
页码范围
2431-2442
出版商
Elsevier
简介
Genome-editing technologies are currently being translated to the clinic. However, cellular effects of the editing machinery have yet to be fully elucidated. Here, we performed global microarray-based gene expression measurements on human CD34+ hematopoietic stem and progenitor cells that underwent editing. We probed effects of the entire editing process as well as each component individually, including electroporation, Cas9 (mRNA or protein) with chemically modified sgRNA, and AAV6 transduction. We identified differentially expressed genes relative to control treatments, which displayed enrichment for particular biological processes. All editing machinery components elicited immune, stress, and apoptotic responses. Cas9 mRNA invoked the greatest amount of transcriptional change, eliciting a distinct viral response and global transcriptional downregulation, particularly of metabolic and cell cycle …
引用总数
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