作者
Juan Guo, Xiaohui Ma, Yuan Cai, Ying Ma, Zhilai Zhan, Yongjin J Zhou, Wujun Liu, Mengxin Guan, Jian Yang, Guanghong Cui, Liping Kang, Lei Yang, Ye Shen, Jinfu Tang, Huixin Lin, Xiaojing Ma, Baolong Jin, Zhenming Liu, Reuben J Peters, Zongbao K Zhao, Luqi Huang
发表日期
2016/4
期刊
New Phytologist
卷号
210
期号
2
页码范围
525-534
简介
- Cytochromes P450 (CYPs) play a key role in generating the structural diversity of terpenoids, the largest group of plant natural products. However, functional characterization of CYPs has been challenging because of the expansive families found in plant genomes, diverse reactivity and inaccessibility of their substrates and products.
- Here we present the characterization of two CYPs, CYP76AH3 and CYP76AK1, which act sequentially to form a bifurcating pathway for the biosynthesis of tanshinones, the oxygenated diterpenoids from the Chinese medicinal plant Danshen (Salvia miltiorrhiza).
- These CYPs had similar transcription profiles to that of the known gene responsible for tanshinone production in elicited Danshen hairy roots. Biochemical and RNA interference studies demonstrated that both CYPs are promiscuous. CYP76AH3 oxidizes ferruginol at two different carbon centers, and CYP76AK1 hydroxylates …
学术搜索中的文章
J Guo, X Ma, Y Cai, Y Ma, Z Zhan, YJ Zhou, W Liu… - New Phytologist, 2016