作者
Jee Loon Foo, Shohei Kitano, Adelia Vicanatalita Susanto, Zhu Jin, Yicong Lin, Zhouqing Luo, Linsen Huang, Zhenzhen Liang, Leslie A Mitchell, Kun Yang, Adison Wong, Yizhi Cai, Jitong Cai, Giovanni Stracquadanio, Joel S Bader, Jef D Boeke, Junbiao Dai, Matthew Wook Chang
发表日期
2023/11/8
期刊
Cell Genomics
卷号
3
期号
11
出版商
Elsevier
简介
Chromosome-level design-build-test-learn cycles (chrDBTLs) allow systematic combinatorial reconfiguration of chromosomes with ease. Here, we established chrDBTL with a redesigned synthetic Saccharomyces cerevisiae chromosome XV, synXV. We designed and built synXV to harbor strategically inserted features, modified elements, and synonymously recoded genes throughout the chromosome. Based on the recoded chromosome, we developed a method to enable chrDBTL: CRISPR-Cas9-mediated mitotic recombination with endoreduplication (CRIMiRE). CRIMiRE allowed the creation of customized wild-type/synthetic combinations, accelerating genotype-phenotype mapping and synthetic chromosome redesign. We also leveraged synXV as a "build-to-learn" model organism for translation studies by ribosome profiling. We conducted a locus-to-locus comparison of ribosome occupancy between synXV …
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JL Foo, S Kitano, AV Susanto, Z Jin, Y Lin, Z Luo… - Cell Genomics, 2023