作者
Lu Ren, Phung Thai, Sruthi Mantri, Lauren Duan, Felix Atzert, Benjamin Beyersdorf, Nipavan Chiamvimonvat, Sean M Wu, William R Goodyer
发表日期
2023/8/4
期刊
Circulation Research
卷号
133
期号
Suppl_1
页码范围
AP3050-AP3050
出版商
Lippincott Williams & Wilkins
简介
Cpne5 (Copine 5) is a Ca2+-dependent phospholipid binding protein implicated in membrane trafficking but has no known role within the heart to date. In single-cell RNA-sequencing of the developing mouse heart, we previously found Cpne5 gene expression to be highly enriched in the cardiac conduction system (CCS). Further, variants in CPNE5 were identified in 6 independent GWAS related to heart rate variability in humans. We therefore hypothesized that Cpne5 is necessary for normal CCS function through Ca2+-dependent membrane trafficking of ion channels.
Whole mount immunostaining with 3D volumetric imaging (iDISCO+) of intact WT hearts demonstrated robust and specific protein expression of Cpne5 throughout all CCS components, including the sinoatrial node (SAN) and SAN-transitional cells. Next, to assess the necessity of Cpne5 within the CCS, systemic knockout mice (Cpne5KO) were …
学术搜索中的文章
L Ren, P Thai, S Mantri, L Duan, F Atzert, B Beyersdorf… - Circulation Research, 2023