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The bio-competitive exclusion (BCX) method is a practical, environment-friendly, and low-cost method of controlling sulfate-reducing bacteria (SRB) and focuses on research and preliminary applications. In this study, a nitrate-reducing bacteria (NRB) were screened and selected from oil-producing water and identified as heterotrophic Pseudomonas sp. through 16S rDNA. The NRB activation and the SRB inhibition systems were studied via the addition of the bio-activators NO₃⁻ and NO₂⁻. In addition, the synergistic inhibitory effect of NRB and molybdate on SRB was studied. Results showed that the inhibitory effect of NO₂⁻ was better than that of NO₃⁻. At NO₃⁻ to NO₂⁻ ratio of 1:4, the inhibitory effect was optimum. The addition of 5% activated NRB and a small amount of molybdic acid salt played a synergistic effect. The optimal inhibition system contained the following: 5% NRB, NO₃⁻/NO₂⁻ of 1:4, and the …