作者
Shui Ping Tu, Jian Tao Cui, Peter Liston, Xiao Hua Jiang, Ruian Xu, Marie CM Lin, Yan Bo Zhu, Bing Zou, Samuel SM Ng, Shi Hu Jiang, Harry HX Xia, Wai Man Wong, Annie OO Chan, Man Fung Yuen, Shiu Kum Lam, Hsiang Fu Kung, Benjamin CY Wong
发表日期
2005/2/1
期刊
Gastroenterology
卷号
128
期号
2
页码范围
361-375
出版商
WB Saunders
简介
Background & Aims
Reactivation of survivin expression is involved in carcinogenesis and angiogenesis in colon cancer. Previous in vitro studies showed that mutation of the cysteine residue at position 84 (Cys84Ala) of survivin generates a dominant-negative mutant that triggers mitotic catastrophe and apoptosis. We investigated the therapeutic effect of the adeno-associated virus (AAV)-mediated survivin mutant (Cys84Ala) on colon cancer.
Methods
Survivin mutant (Cys84Ala) (Sur-Mut(Cys84Ala)) was cloned into the AAV expression vector pAM/CAG-WPRE.poly(A) to generate recombinant AAV-Sur-Mut(Cys84Ala) virus. Cell proliferation, apoptosis, mitotic catastrophe, and tumor growth were measured in vitro and in vivo.
Results
Transduction of colon cancer cells with rAAV-Sur-Mut(Cys84Ala) inhibited cell proliferation and induced apoptosis and mitotic catastrophe in vitro. rAAV-Sur-Mut(Cys84Ala) sensitized …
引用总数
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