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Background

Valproic acid (VPA) and carbamazepine (CBZ), two widely used antiepileptic drugs, have recently been found to inhibit histone deacetylases (HDAC). HDAC inhibitors (HDACIs) have various effects on cancer cells.

Objectives

The aim of this study was to compare the anticancer activity of these drugs on SW480 colon cancer cell lines.

Methods

In the present experimental study, implemented during 2014-2015 in Iran, after incubation of cells into 96-well plates with 5,500 cells/well, the tested drugs were added, and cytotoxic effects were assessed by MTT. Moreover, after incubation of 8× 10 6 cells in 75 cm² flasks to obtain β-catenin levels and 10 6 cells in a six-well plate to obtain vascular endothelial growth factor (VEGF) levels, these levels were estimated using enzyme-linked immunosorbent assay (ELISA) analysis.

Results

Through MTT assay, we found that the inhibitory concentration of 50%(IC50 …