作者
Nihal Dharmasiri, Sunethra Dharmasiri, Mark Estelle
发表日期
2005/5/26
期刊
Nature
卷号
435
期号
7041
页码范围
441-445
出版商
Nature Publishing Group UK
简介
The plant hormone auxin regulates diverse aspects of plant growth and development. Recent studies indicate that auxin acts by promoting the degradation of the Aux/IAA transcriptional repressors through the action of the ubiquitin protein ligase SCFTIR1. The nature of the signalling cascade that leads to this effect is not known. However, recent studies indicate that the auxin receptor and other signalling components involved in this response are soluble factors. Using an in vitro pull-down assay, we demonstrate that the interaction between transport inhibitor response 1 (TIR1) and Aux/IAA proteins does not require stable modification of either protein. Instead auxin promotes the Aux/IAA–SCFTIR1 interaction by binding directly to SCFTIR1. We further show that the loss of TIR1 and three related F-box proteins eliminates saturable auxin binding in plant extracts. Finally, TIR1 synthesized in insect cells binds Aux/IAA …
引用总数
学术搜索中的文章
N Dharmasiri, S Dharmasiri, M Estelle - Nature, 2005