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Stripe rust of wheat, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases of wheat in Canada. This study presents the results from resistance evaluation of Yr genes and western Canadian wheat cultivars from different milling classes, to natural infection in southern Alberta and British Columbia which are considered hot spots of stripe rust occurrence in Canada, due to proximity to Pacific Northwest of the United States where stripe rust epidemics are frequent. Genes Yr1, Yr5, Yr15, and YrSP were effective in all environments; Yr17 and Yr28, which were earlier reported ineffective to existing stripe rust races at the seedling stage in Canada, were effective at adult plant stages in most of the environments because of warmer climates in southerly locations, a favourable condition for expression of the genes. Yr17 is common in winter wheat cultivars and only reported spring wheat …