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Hydrogen peroxide (H₂O₂) is a ubiquitous biological molecule whose wide range of biological functions depends on its concentration. In this chapter, we compare the delivery of H₂O₂ to cells as (1) a single initial dose (bolus addition); (2) a continuous source using, for example, glucose oxidase; and (3) a steady state, in which H₂O₂ concentration is kept constant during the assay. Both the bolus addition and the use of a continuous source of H₂O₂ have as outcome concentration profiles of H₂O₂ that are dependent on experimental conditions and that are difficult to reproduce from the information that is usually revealed in the experimental section of most research articles. On the other hand, the outcome of delivering H₂O₂ as a steady state is a concentration profile that is independent of experimental conditions. The implementation of the steady state starts with the determination of the kinetics of H₂O₂ …