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The present study aimed to determine if betaine can function as a feeding stimulant or feed enhancer for the juvenile grouper, Epinephelus fuscoguttatus through a behavioural experiment with video recording. Agar gel pellet was used as the medium to deliver the chemical test substances – betaine (BET), amino acids mixture (AAM) and mixtures of 1:1, 1:2, 1:3, 1:4, 1:5 BET + AAM to the fish. The pure agar gel (PAG) and feed extract pellets were used as the negative and positive control, respectively. From the recorded videos, two parameters were observed: (I) the pellet was consumed or rejected [A] – if consumed, recorded 1; if rejected, recorded 0, and (II) frequency of the pellet has been captured before it was consumed or rejected and ignored [B], and the preference index was calculated through [A]/[B]. The PAG pellet was totally rejected by the fish (index's value = 0). The index's values of BET and AAM …