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Taste preferences of marble goby (Oxyeleotris marmoratus) for a mixture and several single L-isomer amino acids were determined through behavioral tests. Agar gel pellet was used as the medium to deliver the amino acids, and the fish acceptance on these pellets was evaluated by calculating the acceptance index (total pellet ingested/total pellet given; maximum and minimum values are 1 and 0, respectively). O. marmoratuswere found to moderately accept L-alanine, L-glycine, and L-glutamine (index values 0.38, 0.32, and 0.27, respectively) but reject L-cysteine, L-glutamic acid, L-lysine, L-serine and taurine (index value 0). The amino acids mixture (AAM) showed the highest acceptance (0.91) by the fish. Such results indicated that the fish preferred the amino acids mixture over any of the single amino acids tested. Therefore, the amino acids mixture is potent to function as the feeding stimulant for the O. marmoratus.