查看文章

Methods: A total of 148 consecutive flagged blood culture bottles with Gram-positive cocci were evaluated using iCubate platform in National Taiwan University Hospital. The results of this platform were compared to the final results of combined culture-based MALDI-TOF/MS identification, minimum inhibitory concentrations (oxacillin and vancomycin), 16S rRNA (species identification) and resistance-associated genes sequencing (mecA and vanA/vanB).

Results: The overall sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of this platform for identification of Gram-positive cocci species were 86.7%(65/75), 94.5%(69/73), 94.2%(65/69) and 87.3%(69/79), respectively. For vancomycin-resistant enterococci (VRE) identification, the sensitivity, specificity, PPV, and NPV were 100.0%(10/10), 99.3%(137/138), 90.9%(10/11), and 100.0%(137/137), respectively. For methicillin-resistant …