作者
Wouter R Karthaus, Phillip J Iaquinta, Jarno Drost, Ana Gracanin, Ruben Van Boxtel, John Wongvipat, Catherine M Dowling, Dong Gao, Harry Begthel, Norman Sachs, Robert GJ Vries, Edwin Cuppen, Yu Chen, Charles L Sawyers, Hans C Clevers
发表日期
2014/9/25
期刊
Cell
卷号
159
期号
1
页码范围
163-175
出版商
Elsevier
简介
The prostate gland consists of basal and luminal cells arranged as pseudostratified epithelium. In tissue recombination models, only basal cells reconstitute a complete prostate gland, yet murine lineage-tracing experiments show that luminal cells generate basal cells. It has remained challenging to address the molecular details of these transitions and whether they apply to humans, due to the lack of culture conditions that recapitulate prostate gland architecture. Here, we describe a 3D culture system that supports long-term expansion of primary mouse and human prostate organoids, composed of fully differentiated CK5+ basal and CK8+ luminal cells. Organoids are genetically stable, reconstitute prostate glands in recombination assays, and can be experimentally manipulated. Single human luminal and basal cells give rise to organoids, yet luminal-cell-derived organoids more closely resemble prostate glands …
学术搜索中的文章
WR Karthaus, PJ Iaquinta, J Drost, A Gracanin… - Cell, 2014