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Immobilization of an enzyme can enhance its catalytic activity, depending on the properties of the enzyme and the matrix. Graphene oxide is a nontoxic material and selective modulator for enzyme activity and is also a thermostable molecule that is important in large-scale nanostructure sheet applications. Herein, we have successfully developed a strategy for preparing a nanocomposite for enzyme immobilization model with high loading capacity. Nanostructures of hybrid graphene oxide-Fe₃O₄-cyanuric chloride (GO/MNP-CC) have adjustable surface chemistry that is an excellent candidate for covalent immobilization of enzymes. The morphology, structure and properties of GO/MNP-CC nanocomposite were investigated through different analytical tools. Glucoamylase, an important enzyme in industrial food products, was immobilized on GO/MNP-CC and exhibited excellent catalytic activity at pH 6.5 and 60 °C …