作者
Alex Greenlon, Ella Sieradzki, Olivier Zablocki, Benjamin J Koch, Megan M Foley, Jeffrey A Kimbrel, Bruce A Hungate, Steven J Blazewicz, Erin E Nuccio, Christine L Sun, Aaron Chew, Cynthia-Jeanette Mancilla, Matthew B Sullivan, Mary Firestone, Jennifer Pett-Ridge, Jillian F Banfield
发表日期
2022/10/27
期刊
Msystems
页码范围
e00417-22
出版商
American Society for Microbiology
简介
The growth and physiology of soil microorganisms, which play vital roles in biogeochemical cycling, are shaped by both current and historical soil environmental conditions. Here, we developed and applied a genome-resolved metagenomic implementation of quantitative stable isotope probing (qSIP) with an H218O labeling experiment to identify actively growing soil microorganisms and their genomic capacities. qSIP enabled measurement of taxon-specific growth because isotopic incorporation into microbial DNA requires production of new genome copies. We studied three Mediterranean grassland soils across a rainfall gradient to evaluate the hypothesis that historic precipitation levels are an important factor controlling trait selection. We used qSIP-informed genome-resolved metagenomics to resolve the active subset of soil community members and identify their characteristic ecophysiological traits. Higher …
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A Greenlon, E Sieradzki, O Zablocki, BJ Koch… - Msystems, 2022