查看文章

Purpose: To investigate whether myeloid-derived suppressor cells (MDSCs) in the cornea have neuroregenerative and immunosuppressive action

Methods: Corneal inflammation was induced in Thy1-YFP neurofluorescent homozygous adult mice (8-10week old) with 0.1% Benzalkonium chloride (BAK) eye drops for 1 week. Serial in vivo imaging was performed using a fluorescent stereomicroscope to visualize corneal nerves and infiltrating fluorescent inflammatory cells. YFP+ MDSCs obtained from mouse bone marrows and corneas were used to evaluate their neuroregenerative potential and immunosuppressive action. Increased neurite length in compartmental and transwell culture of trigeminal ganglion (TG) neurites in the presence YFP+ MDSCs, as well as upregulation of specific neurotrophins and regeneration-associated genes were quantified using quantitative PCR and ELISA to evaluate neurotrophic …