作者
N Akhtar, R Hafeez, ZA Awan
发表日期
2014/10
期刊
Plant Disease
卷号
98
期号
10
页码范围
1440-1440
出版商
The American Phytopathological Society
简介
A rice field owned by an individual grower in Lahore, Pakistan, was surveyed in July 2013. Plants with symptoms of black, circular, necrotic spots 3 to 4 mm in diameter and an average of 8 to 10 spots per leaf were observed. Diseased plants were present in the field either singly or in groups of three to five. Ten symptomatic plants were selected randomly, and one infected leaf per plant and one necrotic spot per leaf was selected for the isolation of the pathogen. Necrotic areas were cut into small pieces, surface sterilized with 1% sodium hypochlorite solution, and plated on 2% malt extract agar (MEA) (Sigma, Dorset, UK). After incubation at 25 ± 2°C for 4 to 5 days, fungal mycelium was transferred aseptically to fresh MEA for pure culture. Three different isolates grown for 7 days on MEA were selected for detailed morphological studies. The fungal colony was dark greenish-black, reaching 7 to 8 cm in diameter, with 2 …
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