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Given its coloration, the peel of the infructescence of Ficus carica L. a by-product of fruit processing and/or consumption, was considered as a potential source of anthocyanin compounds. In the present study, different extraction techniques (heat, ultrasound, and microwave), aiming to recover the anthocyanin pigments and optimize its extraction, were compared by response surface methodology using a circumscribed central composite design of three variables with five levels. The relevant tested and optimized variables were time, temperature, and solvent composition (ethanol/water ratio) for heat and microwave extraction methods. For ultrasound method, the variables were the ultrasonic power, time, and solvent composition. The anthocyanin profile of the extract was determined by HPLC-DAD-ESI/MS, and the used criteria responses were the quantification of cyanidin 3-rutinoside (C) in the extracted residue (R; mg C/g R) and in the dried fig peel (P; mg C/g P dw), and the extraction yield of the obtained residue (g R/g P dw). Ultrasound extraction was the most effective method, yielding 17.0 mg C/g R, 3.11 mg C/g P dw, and 0.19 g R/g P dw at the optimal global extraction conditions (21 min, 310 W, and 100% of ethanol). Regarding its bioactive properties, the fig peel optimized extract showed antioxidant activity with an EC50 value of 2,447 mg/mL in the TBARS method and EC80 values of 52 and 1.62 mg/mL, in 60 and 120 min, respectively, in the OxHLIA assay. The extract also revealed antimicrobial activity in different Gram-positive and Gram-negative bacteria and did not present cytotoxicity against non-tumour cells. Its application in a food …