作者
Dustin T Yates, Derek S Clarke, Antoni R Macko, Miranda J Anderson, Leslie A Shelton, Marie Nearing, Ronald E Allen, Robert P Rhoads, Sean W Limesand
发表日期
2014/7/15
期刊
The Journal of physiology
卷号
592
期号
14
页码范围
3113-3125
简介
Key points
- To investigate loss of skeletal muscle mass in intrauterine growth‐restricted (IUGR) fetuses near term, which may result from myoblast dysfunction, we examined semitendinosus myofibre and myoblast morphology in placental insufficiency‐induced IUGR sheep fetuses; we also isolated and cultured IUGR fetal myoblasts to determine whether reduced rates of proliferation were due to intrinsic cellular defects or extrinsic factors associated with serum.
- Using tests for myogenin and pax7 to identify differentiated and undifferentiated fetal myoblasts, respectively, we found that myofibre area and percentage of myogenin‐positive nuclei were less in IUGR fetal semitendinosus muscles than in controls, but myofibre density and percentage of pax7‐positive nuclei were not different.
- The percentage of pax7‐positive cells that expressed proliferating cellular nuclear antigen was less in IUGR semitendinosus …
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DT Yates, DS Clarke, AR Macko, MJ Anderson… - The Journal of physiology, 2014
