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Production of volatile organic compounds (VOCs) is one of the modes of actions of Trichoderma spp. They inhibit pathogenic fungi/bacteria, promote plant growth, and also affect interactions of plants with pathogens. However, the study of VOCs emitted by Trichoderma species associated with olive roots and its benefits are still limited. In the present study, two strains of Trichoderma collected from olive grove soil were evaluated for their antagonistic activity against five strains of Verticillium dahliae named (Vd1 to Vd5) by indirect confrontation assay. In addition, identification and quantification of volatile compounds produced by each strain were assessed through gas chromatography-mass spectrometry (GC–MS). The extraction of metabolites was performed from filtrates using liquid–liquid extraction. Extracts obtained were taken for analysis through GC–MS. Results of indirect confrontation assay showed a high …