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METHODS:

Male and female C57BL6/J mice were used in this study. UCPPS symptoms was induced by intravesical instillations of recombinant mouse vascular endothelial growth factor (VEGF A), an animal mode of UCPPS we previously established. As a read-out of visceral hypersensitivity, Von Frey assay was performed and compared between VEGF-instilled experimental group and saline-instilled control group. Mice were sacrificed after VEGF-induced visceral hypersensitivity was observed, and their L1, L2, L6, S1 and S2 DRGs was harvested for bulk-RNA sequencing.

RESULTS:

Samples were sequenced at a depth of 40 million reads per sample. Data analysis was performed using Basepair Tech platform. Ingenuity pathway analysis (Qiagen) enabled to visualize the biological network changes due to UCPPS. Spliced Transcripts Alignment to a Reference (mm10) revealed changes in the Nociceptor …