作者
M Donà, Alma Balestrazzi, Andrea Mondoni, Graziano Rossi, Lorenzo Ventura, Armando Buttafava, Anca Macovei, MARIA ELISA Sabatini, A Valassi, Daniela Carbonera
发表日期
2013/5/1
期刊
Annals of botany
卷号
111
期号
5
页码范围
987-998
出版商
Oxford University Press
简介
Background and Aims
The germination test currently represents the most used method to assess seed viability in germplasm banks, despite the difficulties caused by the occurrence of seed dormancy. Furthermore, seed longevity can vary considerably across species and populations from different environments, and studies related to the eco-physiological processes underlying such variations are still limited in their depth. The aim of the present work was the identification of reliable molecular markers that might help in monitoring seed deterioration.
Methods
Dry seeds were subjected to artificial ageing and collected at different time points for molecular/biochemical analyses. DNA damage was measured using the RAPD (random amplified polymorphic DNA) approach while the seed antioxidant profile was obtained using both the DPPH (1,1-diphenyl, 2-picrylhydrazyl) assay …
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