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Standardization of artificial fertilization protocols for the European eel, Anguilla anguilla, is a prerequisite for optimizing the use of available gametes in hatchery facilities and for conserving sperm from high quality males, which is either cryopreserved or in living gene banks. The objectives of this research were to provide a rapid, accurate and precise method to quantify sperm density by examining the relationship between sperm density and absorbance by use of a spectrophotometer, determine the optimal number of sperm required to fertilize eggs in a controlled setting, and explore how long eggs are receptive to fertilization post-stripping. Mean sperm density and absorbance at 350 nm were 1.54e + 10 ± 4.95e + 9 sperm/mL and 1.91 ± 0.22 nm, respectively. Regression analysis demonstrated a highly significant positive relationship between sperm density and absorbance using a spectrophotometer at 350 nm (R …