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Here, we report the discovery of a small molecule inhibitor, 2,2′-dihydroxyazobenzene (DAB), of ADP ribosyl cyclase (ADPR-cyclase) and showed that this inhibitor attenuated angiotensin (Ang) II-induced hypertrophic responses.

and results The intracellular concentration of free Ca²⁺ [Ca²⁺]_i in adult rat cardiomyocytes was measured by using a confocal microscope. Cardiac hypertrophy was induced by the two-kidney one-clip (2K1C) method. Hypertrophy was determined by de novo protein synthesis, cell volume, echocardiography, nuclear translocation of nuclear factor of activated T-cells, and transforming growth factor-β1 protein expression. Treatment of cardiomyocytes with Ang II generated a biphasic [Ca²⁺]_i increase that included an initial Ca²⁺peak and sustained Ca²⁺ rise via inositol trisphosphate and cyclic ADP-ribose (cADPR) formation …