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A mixture of three distinct cerium precursors (Ce(NO₃)₃·6H₂O, CeCl₃·7H₂O, and Ce(CH₃COO)₃·H₂O) was used to prepare cerium oxide nanoparticles (CeO₂ NPs) in a polyol-mediated synthesis. Different ratios of diethylene glycol (DEG) and H₂O were utilized in the synthesis. The properties of the synthesized CeO₂ NPs, such as structural and morphological properties, were investigated to observe the effect of the mixed cerium precursors. Crystallite sizes of 7–8 nm were obtained for all samples, and all synthesized samples were confirmed to be in the cubic phase. The average particle sizes of the spherical CeO₂ were between 9 and 13 nm. The successful synthesis of CeO₂ can also be confirmed via the vibrational band of Ce–O from the FTIR. Antidiabetic properties of the synthesized CeO₂ NPs were investigated using α-glucosidase enzyme inhibition assay, and the concentration of the synthesized CeO₂ …