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Recent remarkable advancements in large language models (LLMs) have led to their widespread adoption in various applications. A key feature of these applications is the combination of LLMs with external content, where user instructions and third-party content are combined to create prompts for LLM processing. These applications, however, are vulnerable to indirect prompt injection attacks, where malicious instructions embedded within external content compromise LLM's output, causing their responses to deviate from user expectations. Despite the discovery of this security issue, no comprehensive analysis of indirect prompt injection attacks on different LLMs is available due to the lack of a benchmark. Furthermore, no effective defense has been proposed. In this work, we introduce the first benchmark, BIPIA, to measure the robustness of various LLMs and defenses against indirect prompt injection attacks. Our experiments reveal that LLMs with greater capabilities exhibit more vulnerable to indirect prompt injection attacks for text tasks, resulting in a higher ASR. We hypothesize that indirect prompt injection attacks are mainly due to the LLMs' inability to distinguish between instructions and external content. Based on this conjecture, we propose four black-box methods based on prompt learning and a white-box defense methods based on fine-tuning with adversarial training to enable LLMs to distinguish between instructions and external content and ignore instructions in the external content. Our experimental results show that our black-box defense methods can effectively reduce ASR but cannot completely thwart indirect prompt injection …