作者
Hiroko Endo, Jiro Okami, Hiroaki Okuyama, Toru Kumagai, Junji Uchida, Jumpei Kondo, Tetsuo Takehara, Yasuko Nishizawa, Fumio Imamura, Masahiko Higashiyama, Masahiro Inoue
发表日期
2013/2/1
期刊
Journal of Thoracic Oncology
卷号
8
期号
2
页码范围
131-139
出版商
Elsevier
简介
Introduction
Primary culture of cancer cells is expected to be useful for investigating the biology of cancer and predicting chemosensitivity for individual patients, yet has been hampered by technical difficulties. We recently developed the cancer tissue–originated spheroid (CTOS) method for the primary culture of colorectal cancer cells. In the present study, we applied this system to the primary culture of non–small-cell lung cancer.
Methods
We used 125 surgical specimens and 18 pleural effusions for CTOS preparation. Partially digested tumor fragments were cultured in a medium for embryonic stem cells. CTOSs were subjected to sensitivity assay and signal transduction assay for the epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) erlotinib. We also investigated the effects of growth factors in culturing lung cancer CTOS.
Results
The success rate of CTOS preparation from surgical specimens …
学术搜索中的文章
H Endo, J Okami, H Okuyama, T Kumagai, J Uchida… - Journal of Thoracic Oncology, 2013