作者
Cristina Coscolín, Nadine Katzke, Antonio Garcia-Moyano, José Navarro-Fernández, David Almendral, Monica Martinez-Martinez, Alexander Bollinger, Rafael Bargiela, Christoph Gertler, Tatyana N Chernikova, David Rojo, Coral Barbas, Hai Tran, Olga V Golyshina, Rainhard Koch, Michail M Yakimov, Gro EK Bjerga, Peter N Golyshin, Karl-Erich Jaeger, Manuel Ferrer
发表日期
2019/1/15
期刊
Applied and Environmental Microbiology
卷号
85
期号
2
页码范围
e02404-18
出版商
American Society for Microbiology
简介
Amination of bulky ketones, particularly in (R) configuration, is an attractive chemical conversion; however, known ω-transaminases (ω-TAs) show insufficient levels of performance. By applying two screening methods, we discovered 10 amine transaminases from the class III ω-TA family that were 38% to 76% identical to homologues. We present examples of such enzymes preferring bulky ketones over keto acids and aldehydes with stringent (S) selectivity. We also report representatives from the class III ω-TAs capable of converting (R) and (S) amines and bulky ketones and one that can convert amines with longer alkyl substituents. The preference for bulky ketones was associated with the presence of a hairpin region proximal to the conserved Arg414 and residues conforming and close to it. The outward orientation of Arg414 additionally favored the conversion of (R) amines. This configuration was also found to …
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C Coscolín, N Katzke, A Garcia-Moyano… - Applied and Environmental Microbiology, 2019