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To investigate the uptake and long‐distance translocation of sulphate in plants, we have characterized three cell‐type‐specific sulphate transporters, Sultr1;1, Sultr2;1 and Sultr2;2 in Arabidopsis thaliana. Heterologous expression in the yeast sulphate transporter mutant indicated that Sultr1;1 encodes a high‐affinity sulphate transporter (K_m for sulphate 3.6 ± 0.6 μm), whereas Sultr2;1 and Sultr2;2 encode low‐affinity sulphate transporters (K_m for sulphate 0.41 ± 0.07 m m and ≥ 1.2 m m, respectively). In Arabidopsis plants expressing the fusion gene construct of the Sultr1;1 promoter and green fluorescent protein (GFP), GFP was localized in the lateral root cap, root hairs, epidermis and cortex of roots. β‐glucuronidase (GUS) expressed with the Sultr2;1 promoter was specifically accumulated in the xylem parenchyma cells of roots and leaves, and in the root pericycles and leaf phloem. Expression of …